In the first instance, the determination of the statically required reinforcement (ULS) is independent of the manual specification of the reinforcement areas.
However, if you want to perform the serviceability limit state checks, you have to manually assign the reinforcement to all surfaces selected for design in RF‑CONCRETE Surfaces.
Otherwise, RF‑CONCRETE Surfaces does not apply any reinforcement at the location for the SLS design. The statically required reinforcement is not applied in this case.
In result windows 3.x, a message is displayed (for the unreinforced surfaces) saying that the reinforcement definition is not sufficient. No checks are calculated for these surfaces.